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ウシ胎仔線維芽細胞を核体とした除核成熟卵子との電気融合率に関する検討
http://hdl.handle.net/10470/25893
http://hdl.handle.net/10470/25893e88b1d42-c7da-43c3-8904-12c50c2a3f07
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-08-10 | |||||
タイトル | ||||||
タイトル | ウシ胎仔線維芽細胞を核体とした除核成熟卵子との電気融合率に関する検討 | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
別タイトル | ||||||
その他のタイトル | Examination on the Electric Fusion Rate of the Karyoplast-Cytoplast Complexes Using Bovine Fetal Fibroblast Cells for Donor Cells | |||||
著者名 |
浦川, 真実
× 浦川, 真実× 宇留野, 勝好× 青柳, 敬人× 澤田, 登起彦 |
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著者別名 | ||||||
姓名 | URAKAWA, Manami | |||||
著者別名 | ||||||
姓名 | URUNO, Katsuyoshi | |||||
著者別名 | ||||||
姓名 | AOYAGI, Yoshito | |||||
著者別名 | ||||||
姓名 | SAWADA, Tokihiko | |||||
出版者 | ||||||
出版者 | 東京女子医科大学学会 | |||||
受付日付 | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0040-9022 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00161368 | |||||
書誌情報 |
東京女子医科大学雑誌 巻 71, 号 11/12, p. 823-827, 発行日 2001-12-25 |
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著者版フラグ | ||||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We studied electrofusion of bovine karyoplast-cytoplast complexes in order to define the parameters which would result in a high yield of fused embryos in nuclear transfer, using bovine fibroblast cells. Experiment 1. The effects of the number of electrical pulses (1~20 times) to the cytoplasmic membrane of matured enucleated oocytes in vitro were examined. The number of damaged oocytes increased with higher pulse frequency of 10~20 times (100 V/mm, 60 μsec). Experiment 2. The effects of the number of electrical pulses (1,3 or 5 times) to the fusion rates of cytoplasts (recipient enucleated oocytes) and karyoplasts (donor cells) derived from fetal fibroblast cells were examined. The difference in fusion rate was not significant among the three electrical pulse frequencies. These results demonstrate that the increase of the pulse frequency cause more damage to cytoplasmic membrane while not affecting the fusion rate. | |||||
著者所属 | ||||||
全国農業協同組合連合会ETセンター | ||||||
著者所属 | ||||||
全国農業協同組合連合会ETセンター | ||||||
著者所属 | ||||||
全国農業協同組合連合会ETセンター | ||||||
著者所属 | ||||||
東京女子医科大学腎臓病総合医療センター外科 |