{"created":"2023-06-20T16:59:28.920756+00:00","id":18594,"links":{},"metadata":{"_buckets":{"deposit":"5c64f88f-8ef9-45bc-bedb-32640b7fe488"},"_deposit":{"created_by":3,"id":"18594","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"18594"},"status":"published"},"_oai":{"id":"oai:twinkle.repo.nii.ac.jp:00018594","sets":["140:1307:1308:1956:1964"]},"author_link":["266420","266419","266423","266418","266425","266422","266421","266424"],"item_10001_alternative_title_1":{"attribute_name":"別タイトル","attribute_value_mlt":[{"subitem_alternative_title":"Examination on the Electric Fusion Rate of the Karyoplast-Cytoplast Complexes Using Bovine Fetal Fibroblast Cells for Donor Cells"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2001-12-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11/12","bibliographicPageEnd":"827","bibliographicPageStart":"823","bibliographicVolumeNumber":"71","bibliographic_titles":[{"bibliographic_title":"東京女子医科大学雑誌"}]}]},"item_10001_creator_2":{"attribute_name":"著者名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"浦川, 真実"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"宇留野, 勝好"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"青柳, 敬人"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"澤田, 登起彦"}],"nameIdentifiers":[{}]}]},"item_10001_date_25":{"attribute_name":"受付日付","attribute_value_mlt":[{"subitem_date_issued_datetime":"2010-08-10","subitem_date_issued_type":"Created"}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We studied electrofusion of bovine karyoplast-cytoplast complexes in order to define the parameters which would result in a high yield of fused embryos in nuclear transfer, using bovine fibroblast cells. Experiment 1. The effects of the number of electrical pulses (1～20 times) to the cytoplasmic membrane of matured enucleated oocytes in vitro were examined. The number of damaged oocytes increased with higher pulse frequency of 10～20 times (100 V/mm, 60 μsec). Experiment 2. The effects of the number of electrical pulses (1,3 or 5 times) to the fusion rates of cytoplasts (recipient enucleated oocytes) and karyoplasts (donor cells) derived from fetal fibroblast cells were examined. The difference in fusion rate was not significant among the three electrical pulse frequencies. These results demonstrate that the increase of the pulse frequency cause more damage to cytoplasmic membrane while not affecting the fusion rate.","subitem_description_type":"Abstract"}]},"item_10001_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"266422","nameIdentifierScheme":"WEKO"}],"names":[{"name":"URAKAWA, Manami"}]},{"nameIdentifiers":[{"nameIdentifier":"266423","nameIdentifierScheme":"WEKO"}],"names":[{"name":"URUNO, Katsuyoshi"}]},{"nameIdentifiers":[{"nameIdentifier":"266424","nameIdentifierScheme":"WEKO"}],"names":[{"name":"AOYAGI, Yoshito"}]},{"nameIdentifiers":[{"nameIdentifier":"266425","nameIdentifierScheme":"WEKO"}],"names":[{"name":"SAWADA, Tokihiko"}]}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"東京女子医科大学学会"}]},"item_10001_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AN00161368","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0040-9022","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_35":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"全国農業協同組合連合会ETセンター"},{"subitem_text_value":"全国農業協同組合連合会ETセンター"},{"subitem_text_value":"全国農業協同組合連合会ETセンター"},{"subitem_text_value":"東京女子医科大学腎臓病総合医療センター外科"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-03-27"}],"displaytype":"detail","filename":"KJ00006020068.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00006020068.pdf","url":"https://twinkle.repo.nii.ac.jp/record/18594/files/KJ00006020068.pdf"},"version_id":"c3c8a6ac-7f40-4870-9e41-4876f7e30aef"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ウシ胎仔線維芽細胞を核体とした除核成熟卵子との電気融合率に関する検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ウシ胎仔線維芽細胞を核体とした除核成熟卵子との電気融合率に関する検討"}]},"item_type_id":"10001","owner":"3","path":["1964"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-08-10"},"publish_date":"2010-08-10","publish_status":"0","recid":"18594","relation_version_is_last":true,"title":["ウシ胎仔線維芽細胞を核体とした除核成熟卵子との電気融合率に関する検討"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-20T22:26:18.169693+00:00"}