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培養ヒト腎糸球体臓側上皮細胞の継代培養に関する基礎的検討
http://hdl.handle.net/10470/23865
http://hdl.handle.net/10470/2386561c76ccb-3c96-47b9-9894-7988f31f2f95
名前 / ファイル | ライセンス | アクション |
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KJ00006025123.pdf (1.5 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-08-10 | |||||
タイトル | ||||||
タイトル | 培養ヒト腎糸球体臓側上皮細胞の継代培養に関する基礎的検討 | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
別タイトル | ||||||
その他のタイトル | A Basic Study in Culturing Human Glomerular Visceral Epithelial Cells | |||||
著者名 |
筒井, 貴朗
× 筒井, 貴朗× 新田, 孝作× 湯村, 和子× 二瓶, 宏 |
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著者別名 | ||||||
姓名 | TSUTSUI, Takaaki | |||||
著者別名 | ||||||
姓名 | NITTA, Kosaku | |||||
著者別名 | ||||||
姓名 | YUMURA, Wako | |||||
著者別名 | ||||||
姓名 | NIHEI, Hiroshi | |||||
出版者 | ||||||
出版者 | 東京女子医科大学学会 | |||||
受付日付 | ||||||
日付 | 2010-08-10 | |||||
日付タイプ | Created | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0040-9022 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00161368 | |||||
書誌情報 |
東京女子医科大学雑誌 巻 67, 号 7, p. 457-464, 発行日 1997-07-25 |
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著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Visceral glomerular epithelial cells (v-GECs) are highly differentiated and specialized cells which form an important component of the glomerular filtration barrier. As v-GECs are highly differentiated cells, it is not elucidated whether v-GECs have the capacity to proliferate and whether GEC grown in culture are of visceral or parietal origin. We found this difference to be related to difficulty in culturing GEC beyond the 6th passage. With immunocytochemical methods, the cells which were difficult to culture were found to possess characteristics of v-GECs. In these cells, we investigated the mechanisms of cell death, particularly the appearance of apoptosis. Under serum free conditions, v-GECs degenerated in a time dependent manner. With flow cytometric analysis and agarose gel electrophoresis, cell death under serum free conditions and after 6th passages depended on apoptotic mechanisms based on the demonstration of DNA fragmentation. The addition of heparin and protein kinase C inhibitors, H-7 and staurosporin, suppressed cell degeneration and DNA fragmentation. It was concluded that v-GECs could not be cultured beyond the 6th passage due to apoptotic mechanisms and the development of apoptosis appeared to require the activation of protein kinase C. | |||||
著者所属 | ||||||
東京女子医科大学第四内科学教室 | ||||||
著者所属 | ||||||
東京女子医科大学第四内科学教室 | ||||||
著者所属 | ||||||
東京女子医科大学第四内科学教室 | ||||||
著者所属 | ||||||
東京女子医科大学第四内科学教室 |