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ベーチェット病患者における多核白血球の補体第3成分(C3)産生能
http://hdl.handle.net/10470/23714
http://hdl.handle.net/10470/23714784e5d36-53ae-4d1f-bc5c-084552256ded
名前 / ファイル | ライセンス | アクション |
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KJ00006024946.pdf (900.6 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-08-10 | |||||
タイトル | ||||||
タイトル | ベーチェット病患者における多核白血球の補体第3成分(C3)産生能 | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
別タイトル | ||||||
その他のタイトル | Production of the Third Component of Complement (C3) by Polymorphonuclear Leukocytes from Patients with Behcet's Disease | |||||
著者名 |
陳, 麗理
× 陳, 麗理 |
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著者別名 | ||||||
姓名 | CHEN, Li-Li | |||||
出版者 | ||||||
出版者 | 東京女子医科大学学会 | |||||
受付日付 | ||||||
日付 | 2010-08-10 | |||||
日付タイプ | Created | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0040-9022 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00161368 | |||||
書誌情報 |
東京女子医科大学雑誌 巻 67, 号 1/2, p. 33-42, 発行日 1997-02-25 |
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著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | To elucidate the relationship between the functions of polymorphonuclear leukocytes (PMN) and the complement system in patients with Behcet's disease, we analyzed peripheral PMN and the following phenomena were assessed: (1) production of the third component of complement (C3), (2) relationship between secretion and expression of mRNA of C3, (3) effect of 12-o-tetradecanoylphorbol 13-acetate (TPA) on C3 production. The PMN samples were purified more than 98% by the double gradient method and were incubated for 24 hours. C3 levels in culture supernatants were quantitated by sandwich enzyme-linked immunosorbent assay, and C3 mRNA were detected by a reverse transcription-polymerase chain reaction assay and Southern blot hybridization. C3 secretion by Behcet's PMN was significantly higher than that of normal PMN, though the expression of C3 mRNA by Behcet's PMN was almost the same as that of normal PMN. In the Behcet's PMN group, C3 secretion is closely related to the time-course of ocular attack. When TPA was added to the culture supernatant, C3 secretion and C3 mRNA expression by normal PMN were somewhat increased, while there were no changes in the Behcet's PMN. These observations indicate that Behcet's PMN are primed in vivo and suggest that C3 produced by PMN plays a role in the pathophysiology of Behcet's disease. | |||||
著者所属 | ||||||
東京女子医科大学眼科学教室 |