{"created":"2023-06-20T17:02:40.790046+00:00","id":22766,"links":{},"metadata":{"_buckets":{"deposit":"2c7427be-321d-4fca-b9a9-9d9553bb97bb"},"_deposit":{"created_by":3,"id":"22766","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"22766"},"status":"published"},"_oai":{"id":"oai:twinkle.repo.nii.ac.jp:00022766","sets":["140:168:174:175","176:1122:1123","176:1182:1183"]},"author_link":["279487","279488","279489"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"182","bibliographicPageStart":"179","bibliographicVolumeNumber":"22","bibliographic_titles":[{"bibliographic_title":"Journal of analytical atomic spectrometry"}]}]},"item_10001_creator_2":{"attribute_name":"著者名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"MIYAYAMA, Takamitsu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OGRA, Yasumitsu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SUZUKI, T.　Kazuo"}],"nameIdentifiers":[{}]}]},"item_10001_date_25":{"attribute_name":"受付日付","attribute_value_mlt":[{"subitem_date_issued_datetime":"2013-12-20","subitem_date_issued_type":"Created"}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A two-dimensional (2D) micro HPLC-inductively coupled plasma mass spectrometry (ICP-MS) system consisting of a gel filtration column and an anion-exchange column was constructed to separate two metallothionein (MT) isoforms. MT-I and MT-II genes were specifically knocked down with the RNA interference (RNAi) technique, and the effects of the knockdown were evaluated by the reverse transcription-polymerase chain reaction (RT-PCR) and the 2D micro HPLC-ICP-MS system. The minimum cell number required by our 2D micro HPLC-ICP-MS system was calculated to be 2.0 x 10(3), and the detection limit of MT protein was estimated to be 22.2 mg L-1.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"RSC Publishing"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1039/B613662c|10.1039/B613662c","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"著作権関連情報","attribute_value_mlt":[{"subitem_rights":"© Royal Society of Chemistry 2013"}]},"item_10001_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA10716241","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0267-9477","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_35":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"衛生学公衆衛生学（一）"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-03-27"}],"displaytype":"detail","filename":"R131025160505557242.pdf","filesize":[{"value":"481.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"R131025160505557242.pdf","url":"https://twinkle.repo.nii.ac.jp/record/22766/files/R131025160505557242.pdf"},"version_id":"40ac85ec-9a4f-4895-a22f-cf4d07b553bf"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Separation of metallothionein isoforms extracted from isoform-specific knockdown cells on two-dimensional micro high-performance liquid chromatography hyphenated with inductively coupled plasma-mass spectrometry","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Separation of metallothionein isoforms extracted from isoform-specific knockdown cells on two-dimensional micro high-performance liquid chromatography hyphenated with inductively coupled plasma-mass spectrometry"}]},"item_type_id":"10001","owner":"3","path":["175","1123","1183"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-12-20"},"publish_date":"2013-12-20","publish_status":"0","recid":"22766","relation_version_is_last":true,"title":["Separation of metallothionein isoforms extracted from isoform-specific knockdown cells on two-dimensional micro high-performance liquid chromatography hyphenated with inductively coupled plasma-mass spectrometry"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T20:31:02.327247+00:00"}