{"created":"2023-06-20T17:00:08.355364+00:00","id":19442,"links":{},"metadata":{"_buckets":{"deposit":"2a4f6f24-8a6a-46e3-b327-1f9915a7e949"},"_deposit":{"created_by":3,"id":"19442","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"19442"},"status":"published"},"_oai":{"id":"oai:twinkle.repo.nii.ac.jp:00019442","sets":["140:1307:1308:1984:1994"]},"author_link":["293734","293730","293728","293733","293735","293731","293726","293727","293732","293729"],"item_10001_alternative_title_1":{"attribute_name":"別タイトル","attribute_value_mlt":[{"subitem_alternative_title":"Analysis of Klotho Expression in the Mouse Kidney Cell Line"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageEnd":"679","bibliographicPageStart":"673","bibliographicVolumeNumber":"74","bibliographic_titles":[{"bibliographic_title":"東京女子医科大学雑誌"}]}]},"item_10001_creator_2":{"attribute_name":"著者名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"三戸部, 倫大"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"杉浦, 秀和"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"芳田, 工"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"土谷, 健"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"二瓶, 宏"}],"nameIdentifiers":[{}]}]},"item_10001_date_25":{"attribute_name":"受付日付","attribute_value_mlt":[{"subitem_date_issued_datetime":"2010-08-10","subitem_date_issued_type":"Created"}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Defects in klotho gene expression in the mouse result in a syndrome that resembles human aging. However, the precise properties of klotho protein, the mechanism regulating its expression, etc., have not been clarified. First, using specific antibody we identified klotho expression in mouse kidney as mainly localizing in the distal and collecting duct cells. Based on this information we examined the expression of klotho in a murine inner medullary collecting duct cell line (mIMCD3). We then explored the physiological relevance of regulation of klotho expression under oxidative stress condition. Expression of klotho in mIMCD3 cells was identified by real-time PCR, immunoprecipitation-Western blotting, and immunocytochemical staining. Cells were transfected with recombinant klotho adenovirus and overexpression of klotho gene was induced to confirm the expression of internal klotho. Oxidative stress injury produced by adding hydrogen peroxide to the culture medium dose-dependently reduced klotho expression maximally 24 hours, and it also diminished klotho staining. The results of this study demonstrated that klotho expressed in cultured mIMCD3 cells and that the klotho gene may be involved in the process of oxidative stress injury in these cells.","subitem_description_type":"Abstract"}]},"item_10001_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"293731","nameIdentifierScheme":"WEKO"}],"names":[{"name":"MITOBE, Michihiro"}]},{"nameIdentifiers":[{"nameIdentifier":"293732","nameIdentifierScheme":"WEKO"}],"names":[{"name":"SUGIURA, Hidekazu"}]},{"nameIdentifiers":[{"nameIdentifier":"293733","nameIdentifierScheme":"WEKO"}],"names":[{"name":"YOSHIDA, Takumi"}]},{"nameIdentifiers":[{"nameIdentifier":"293734","nameIdentifierScheme":"WEKO"}],"names":[{"name":"TSUCHIYA, Ken"}]},{"nameIdentifiers":[{"nameIdentifier":"293735","nameIdentifierScheme":"WEKO"}],"names":[{"name":"NIHEI, Hiroshi"}]}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"東京女子医科大学学会"}]},"item_10001_relation_17":{"attribute_name":"関連文献URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://hdl.handle.net/10470/26785"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://hdl.handle.net/10470/26785","subitem_relation_type_select":"HDL"}}]},"item_10001_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AN00161368","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0040-9022","subitem_source_identifier_type":"ISSN"}]},"item_10001_subject_47":{"attribute_name":"著者キーワード","attribute_value_mlt":[{"subitem_subject":"adenovirus","subitem_subject_scheme":"Other"},{"subitem_subject":"collecting duct","subitem_subject_scheme":"Other"},{"subitem_subject":"hydrogen peroxide","subitem_subject_scheme":"Other"},{"subitem_subject":"klotho","subitem_subject_scheme":"Other"},{"subitem_subject":"oxidative stress","subitem_subject_scheme":"Other"}]},"item_10001_text_35":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東京女子医科大学医学部第四内科学"},{"subitem_text_value":"東京女子医科大学医学部第四内科学"},{"subitem_text_value":"東京女子医科大学医学部第四内科学"},{"subitem_text_value":"東京女子医科大学医学部第四内科学"},{"subitem_text_value":"東京女子医科大学医学部第四内科学"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-08-22"}],"displaytype":"detail","filename":"KJ00006019085.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00006019085.pdf","url":"https://twinkle.repo.nii.ac.jp/record/19442/files/KJ00006019085.pdf"},"version_id":"60af9ea4-a6f6-4b9d-9dcd-11848fd787f4"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"マウス腎由来培養細胞におけるklothoの発現検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"マウス腎由来培養細胞におけるklothoの発現検討"}]},"item_type_id":"10001","owner":"3","path":["1994"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-08-10"},"publish_date":"2010-08-10","publish_status":"0","recid":"19442","relation_version_is_last":true,"title":["マウス腎由来培養細胞におけるklothoの発現検討"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-20T21:03:55.771060+00:00"}