@article{oai:twinkle.repo.nii.ac.jp:00018275,
 author = {CUI, Sanzhe and MARUOKA, Yasubumi and ANDO, Tomohiro and KOBAYASHI, Makio and OGIUCHI, Hideki},
 issue = {1},
 journal = {東京女子医科大学雑誌},
 month = {Jan},
 note = {EBVはヒトに極めて広範に常在するヘルペスウイルスであり,バーキットリンパ腫,上咽頭癌の病因ウイルスと考えられる.口腔領域においては免疫不全者の舌白板症,唾液腺腫瘍への関連が指摘されている.今回,我々は当科で治療を行った口腔腫瘍および粘膜疾患105例について,疾患とEBV感染の関連を検討するため,PCR法,PCR-Southern blot hybridization法,PNA probeを使用したin situ hybridization (ISH)法でEBVゲノムの検出およびmRNAの局在の検討を行った.PCRでは口腔扁平上皮癌18/22例,線維腫11/21例,epulis 16/19例,白板症8/16例,多形性腺腫6/10例,乳頭腫6/9例,口腔扁平苔癬7/8例でEBVゲノムが検出され,EBV DNAの発現率は68.5%であった.ISHではEBVのEBERおよびBHLF mRNAの発現部位は主にリンパ球に観察された.EBERとBHLF陽性細胞の局在は調べた疾患によってまちまちであったが,口腔扁平苔癬にはEBERおよびBHLF陽性リンパ球のlabelling indexが他の疾患に比較して有意に高値を示した.以上の結果より口腔組織中にEBVが高率に存在し,EBV感染細胞の局在は主にリンパ球であり,口腔扁平苔癬の病因にEBV感染が関与している可能性が示唆された., To determine the role of the Epstein-Barr virus (EBV) in the pathogenesis of oral diseases, 105 formalin-fixed, paraffin-embeded specimens were examined. These specimens included 22 cases of oral squamous cell carcinoma, 21 cases of fibroma, 19 cases of epulis, 16 cases of oral leukoplakia, 10 cases of plemorphic adenoma, 9 cases of oral papilloma and 8 cases of oral lichen planus (OLP). The specimens were analyzed using for polymerase chain reaction (PCR), PCR-Southern blot hybridization, and in situ hybridization (ISH) with peptid nucleic acid probes for EBV-encoded small RNAs (EBER) and immediate early mRNAs of the BamH fragment of lower strand frame (BHLF). PCR products for EBV DNA were detected in 68.5% of the examined specimens. In the ISH analysis, EBER and BHLF hybridization signals were mainly localized in the infiltrating lymphocytes. Although the proportion of EBER or BHLF positive lymphocytes varied among the examined diseases, OLP specimens expressed a significantly high level of EBER or BHLF. These results suggest that EBV infection may be implicated in the pathogenesis of OLP.},
 pages = {7--15},
 title = {Role of Epstein-Barr Virus Infection in Oral Diseases},
 volume = {71},
 year = {2001}
}