@article{oai:twinkle.repo.nii.ac.jp:00016875,
 author = {NAKAYAMA, Setsuko and IWASHITA, Mitsutoshi and TAKEDA, Yoshihiko},
 issue = {12},
 journal = {東京女子医科大学雑誌},
 month = {Dec},
 note = {IGF-1およびIGFBP-1の胎児発育に及ぼす生物学的特徴を検討するために,臍帯血中IGF-1およびIGFBP-1をIRMAおよびELISAで測定した.臍帯血中IGF-1のレベルは妊娠週数とともに増加し,またpercentile birth weightとも正の相関を示したのに対し,IGFBP-1の臍帯血中濃度は妊娠週数とともに減少し,percentile birth weightとも逆相関した.これらのことより,IGFBP-1は,胎児発育に負に働いていると考えられた.IGFBP-1を含むIGF結合蛋白の動態を更に臍帯血のWestern ligand blotを用いて分析した.正期産の正常発育児(appropriate-for-gestational-age (AGA)児)の臍帯血中には42/39, 34, 30, 24kDaの4つのIGFに対する結合活性が認められた.これらはそれぞれ,IGFBP-3, IGFBP-2, IGFBP-1, IGFBP-4と考えられた.これに対して,正期産の胎内発育遅延児(light-for-gestational-age (LGA)児)では,IGFBP-3の減少と,IGFBP-2の増加が認められた.逆に,糖尿病を合併した母体から生まれた正期産巨大児(heavy-for-gestational-age (HGA)児)ではIGFBP-3の増加とIGFGBP-2, IGFBP-1の減少を認めた.早産LGA児の臍帯血中においては,早産児AGAと比較してIGFBP-2が著明に増加していた.これらの結果から,IGF-1だけではなく,IGFBPもIGFの活性を修飾することによって胎児発達に重要な役割を果たしていることが示唆された., To evaluate physiological significance of insulin-like growth factor-I (IGF-I) and its binding proteins (IGFBPs), IGF-I and IGFBP-1 in cord sera were measured by immunoradiometeric assay (IRMA) and enzyme-linked immunosorbent assay (ELISA), respectively. Furthermore, IGFBPs in cord sera were analyzed by Western ligand blot. The levels of IGF-I in cord sera increased along with gestation and correlated with birth weight. In contrast, the levels of IGFBP-1 decreased with gestation and inversely correlated with birth weight. Western ligand blot revealed molecular forms of 42/39 kDa doublet, 34 kDa, 30 kDa and 24 kDa IGFBPs corresponding to IGFBP-3, IGFBP-2, IGFBP-1, and IGFBP-4, respectively. Term fetuses with light-for-gestational-age (LGA) revealed marked decrease in IGFBP-3 band with increase in IGFBP-2 band compared to those in fetuses with appropriate-for-gestational-age (AGA). In contrast, term fetuses with heavy-for-gestational-age (HGA) from mothers with diabetes mellitus showed increased IGFBP-3 with decreased IGFBP-2 and IGFBP-1. In preterm fetuses, IGFBP-2 was markedly increased especially in fetuses with LGA compared to AGA at preterm. These results suggest that IGF-I and IGFBPs reflect to growth and developmental stages of fetuses and may have important roles in fetal development.},
 pages = {998--1005},
 title = {INSULIN-LIKE GROWTH FACTOR-I AND ITS BINDING PROTEINS IN CORD SERA : PHYSIOLOGICAL ROLES IN FETAL GROWTH},
 volume = {67},
 year = {1997}
}