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アイテム
マウス褐色および白色脂肪細胞におけるレプチン分泌とレプチンmRNA発現に及ぼすアンジオテンシンIIの作用
http://hdl.handle.net/10470/25055
http://hdl.handle.net/10470/250556845498e-1dec-4e29-8d73-edbfa8fc9940
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00006020143.pdf (706.4 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-08-10 | |||||
| タイトル | ||||||
| タイトル | マウス褐色および白色脂肪細胞におけるレプチン分泌とレプチンmRNA発現に及ぼすアンジオテンシンIIの作用 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 別タイトル | ||||||
| その他のタイトル | Differential Effects of Angiotensin II on the Secretion and mRNA Expression of Leptin in Mouse Cultured Brown and White Adipocytes | |||||
| 著者名 |
三品, 直子
× 三品, 直子× 内田, 庸子× 成瀬, 光栄× 村木, 篁× 高野, 加寿恵 |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 263463 | |||||
| 姓名 | MISHINA, Naoko | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 263464 | |||||
| 姓名 | UCHIDA, Yoko | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 263465 | |||||
| 姓名 | NARUSE, Mitsuhide | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 263466 | |||||
| 姓名 | MURAKI, Takamura | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 263467 | |||||
| 姓名 | TAKANO, Kazue | |||||
| 出版者 | ||||||
| 出版者 | 東京女子医科大学学会 | |||||
| 受付日付 | ||||||
| 日付 | 2010-08-10 | |||||
| 日付タイプ | Created | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0040-9022 | |||||
| NCID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00161368 | |||||
| 書誌情報 |
東京女子医科大学雑誌 巻 70, 号 3, p. 48-56, 発行日 2000-03-25 |
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| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Although it has been implied that the renin/angiotensin system is deeply involved in the regulation of adipocyte function, the details of the role of the system remain to be elucidated. In the present study, we investigated the effects of angiotensin II (Ang II) on leptin secretion and leptin mRNA expression in the adipocytes of brown adipose tissues (BAT) and white adipose tissues (WAT) of mice. Mature adipocytes were prepared by collagenase treatment and culture in DMEM containing 10% fetal bovine serum and 4 nM insulin. Cultured adipocytes were incubated in the medium containing Ang II (10^<-9>～10^<-11>M),the AT1-selective antagonist Losartan (10^<-6>～10^<-8>M), or the AT2-selective antagonist PD 123319 (10^<-6>～10^<-8>M) alone, or a combination of Ang II and antagonist. After incubation for 24 hr, the leptin concentration in the medium was determined by radioimmunoassay. Leptin mRNA expression in the cells was determined by RT-PCR. Ang II increased and decreased leptin secretion by BAT and WAT, respectively, in a dose-dependent manner. Ang II did not affect leptin mRNA expression in BAT, but it significantly decreased leptin mRNA expression in WAT. The effect of Ang II on leptin secretion by BAT was significantly suppressed by PD123319 and the effect of Ang II on leptin secretion and leptin mRNA expression in WAT was suppressed by Losartan. These results suggested that Ang II is involved in the regulation of adipose tissue function through its effects on leptin synthesis and/or secretion. In addition, since the effects in BAT and WAT were mediated through different Ang II receptor subtypes, AT2 in BAT and AT1 in WAT, it is suggested that the receptor subtype is of great biological significance in the action of Ang II on adipocytes as well as on the cardiovascular system. | |||||
| 著者所属 | ||||||
| 値 | 東京女子医科大学医学部第二内科学 | |||||
| 著者所属 | ||||||
| 値 | 東京女子医科大学医学部第二内科学 | |||||
| 著者所属 | ||||||
| 値 | 東京女子医科大学医学部第二内科学 | |||||
| 著者所属 | ||||||
| 値 | 東京女子医科大学医学部薬理学 | |||||
| 著者所属 | ||||||
| 値 | 東京女子医科大学医学部第二内科学 | |||||
