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Separation of metallothionein isoforms extracted from isoform-specific knockdown cells on two-dimensional micro high-performance liquid chromatography hyphenated with inductively coupled plasma-mass spectrometry
http://hdl.handle.net/10470/30321
http://hdl.handle.net/10470/303218349b8be-fb95-411e-9c5e-9bc527312306
名前 / ファイル | ライセンス | アクション |
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R131025160505557242.pdf (481.0 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-12-20 | |||||
タイトル | ||||||
タイトル | Separation of metallothionein isoforms extracted from isoform-specific knockdown cells on two-dimensional micro high-performance liquid chromatography hyphenated with inductively coupled plasma-mass spectrometry | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者名 |
MIYAYAMA, Takamitsu
× MIYAYAMA, Takamitsu× OGRA, Yasumitsu× SUZUKI, T. Kazuo |
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出版者 | ||||||
出版者 | RSC Publishing | |||||
受付日付 | ||||||
日付 | 2013-12-20 | |||||
日付タイプ | Created | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0267-9477 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10716241 | |||||
書誌情報 |
Journal of analytical atomic spectrometry 巻 22, 号 2, p. 179-182, 発行日 2007-02 |
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DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1039/B613662c|10.1039/B613662c | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A two-dimensional (2D) micro HPLC-inductively coupled plasma mass spectrometry (ICP-MS) system consisting of a gel filtration column and an anion-exchange column was constructed to separate two metallothionein (MT) isoforms. MT-I and MT-II genes were specifically knocked down with the RNA interference (RNAi) technique, and the effects of the knockdown were evaluated by the reverse transcription-polymerase chain reaction (RT-PCR) and the 2D micro HPLC-ICP-MS system. The minimum cell number required by our 2D micro HPLC-ICP-MS system was calculated to be 2.0 x 10(3), and the detection limit of MT protein was estimated to be 22.2 mg L-1. | |||||
著作権関連情報 | ||||||
権利情報 | © Royal Society of Chemistry 2013 | |||||
著者所属 | ||||||
衛生学公衆衛生学(一) |