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Mitochondrial electron transport is inhibited by disappearance of metallothionein in human bronchial epithelial cells following exposure to silver nitrate
http://hdl.handle.net/10470/30315
http://hdl.handle.net/10470/303153e3d9082-afa1-49e1-ab3f-e0dcbea05bc7
名前 / ファイル | ライセンス | アクション |
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R131025155602557235.pdf (1.1 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-12-20 | |||||
タイトル | ||||||
タイトル | Mitochondrial electron transport is inhibited by disappearance of metallothionein in human bronchial epithelial cells following exposure to silver nitrate | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者名 |
MIYAYAMA, Takamitsu
× MIYAYAMA, Takamitsu× ARAI, Yuta× SUZUKI, Noriyuki× HIRANO, Seishiro |
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出版者 | ||||||
出版者 | Elsevier | |||||
受付日付 | ||||||
日付 | 2013-12-20 | |||||
日付タイプ | Created | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1879-3185 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0300-483X | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00864402 | |||||
書誌情報 |
Toxicology 巻 305, p. 20-29, 発行日 2013-03 |
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PMID | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 23333424 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.tox.2013.01.004S0300-483X(13)00009-7 [pii] | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Silver (Ag) possesses antibacterial activity and has been used in wound dressings and deodorant powders worldwide. However, the metabolic behavior and biological roles of Ag in mammals have not been well characterized. In the present study, we exposed human bronchial epithelial cells (BEAS-2B) to AgNO3 and investigated uptake and intracellular distribution of Ag, expression of metallothionein (MT), generation of reactive oxygen species (ROS), and changes in mitochondrial respiration. The culture medium concentration of Ag decreased with time and stabilized at 12h. The concentration of both Ag and MT in the soluble cellular fraction increased up to 3h and then decreased, indicating that cytosolic Ag relocated to the insoluble fraction of the cells. The levels of mRNAs for the major human MT isoforms MT-I and MT-II paralleled with the protein levels of Ag-MT. The intensity of fluorescence derived from ROS was elevated in the mitochondrial region at 24h. Ag decreased mitochondrial oxygen consumption in a dose-dependent manner and the activity of mitochondrial complex I-IV enzymes was significantly inhibited following exposure to Ag. In a separate experiment, we found that hydrogen peroxide (H2O2) at concentrations as low as 0.001% (equivalent to the concentration of H2O2 in Ag-exposed cells) removed Ag from MT. These results suggest MT was decomposed by cytosolic H2O2, and then Ag released from MT relocated to insoluble cellular fractions and inhibited electron chain transfer of mitochondrial complexes, which eventually led to cell damage. | |||||
著作権関連情報 | ||||||
権利情報 | Crown copyright © 2013 Published by Elsevier Ireland Ltd. All rights reserved. | |||||
著者所属 | ||||||
衛生学公衆衛生学(一) |