To elucidate the precise mechanisms of low responsiveness to hepatitis B vaccine, we first examined the in vitro hepatitis B surface antigen (HBsAg)-induced proliferation of peripheral blood mononuclear cells (PBMC) obtained from HB vaccines. PBMC obtained from HB vaccine responders tended to respond well to HBsAg, whereas those from either low or non-responders did not. Furthermore, by the depletion of CD8 cells from PBMC, in vitro proliferative activity was restored in three low responders. Using PBMC of two HB vaccine low responders, we successfully established several different CD4 clones and found that at least four different T cells receptor β chain variable gene using clones existed. By the analysis of the synthesis of various cytokine form these clones, we demonstrated that both Th1 and Th2 type CD4 clones existed in these clones. In addition, we were able to establish CD8 Ts clones having an ability to suppress the function of HBsAg-reactive CD4 clones. Thus, our results indicate that while several HBsAg-reactive CD4 clones existed in HB vaccine low responders their function was suppressed by CD8 T cells.
HBワクチン非応答性の解析(免疫学の進歩-基礎と臨床-,シンポジウム,東京女子医科大学学会第308回例会)
KJ00006025073.pdf
(413.32KB)
Twinkleトップページ
